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Fig. 2 AH in cardiac contraction is noted in adult zebrafish heterozygous ttntv mutants. (A–C) Quantification of the ejection fraction in adult zebrafish at 3–12 months via high-frequency echocardiography. One-way ANOVA was applied. (D and E) Quantification of end-systolic volume/body weight (ESV/BW) in adult zebrafish at 6–12 months. One-way ANOVA was applied. (F) Comparison of dissected ventricular size in 6-month fish. Independent samples t test was applied to compare WT with dZ/+ and WT with dA/+. (G) Expression of nppa, nppb, vmhc, and vmhcl gene transcripts by quantitative real-time PCR in 6-month WT, dZ/+, and dA/+ fish. One-way ANOVA was applied. (H) The swimming challenge test indicated a reduced swimming ability in ttntv fish compared with WT fish at 15 months of age. Two-way ANOVA was applied to compare WT versus dZ/+, WT versus dA/+, and dZ/+ versus dA/+ in each day group. Data are presented as mean ± SD. VSA, ventricular surface area; BL, body length.