Figure 11

Figure 11

Analysis of somatic cell apoptosis in wild-type, cdc27^−/−, cdkn1a knockout, and tp53 knockout zebrafish embryos at 24 h post-fertilization (hpf), 48 hpf, and 72 hpf. This figure displays the results of TUNEL staining, a method used to detect apoptosis in somatic cells, across different groups of embryos at various developmental stages. In the cdc27^−/− mutant embryos, TUNEL staining signals, indicative of apoptotic cells, are observed in the head, trunk, spine, encephalocoele, and ventricle regions at 24, 48, and 72 hpf, respectively. In contrast, control siblings and embryos with cdkn1a or tp53 knockouts show no apparent apoptotic signals. The dotted boxes highlight areas of TUNEL staining, while the solid boxes provide enlarged views of these regions, emphasizing the specific areas where apoptosis is detected in the mutants.