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Fig. 2 Effects of pou5f3 mutation and en-pou5f3 induction on the expression of genes regulating posterior development. (A–L) Expression of regulatory genes in the tail region in pou5f3 mutants at 18 hpf. For each gene examined, wild-type/heterozygote (+/−) and homozygote (−/−) embryos are shown at the top and bottom, respectively. (M–P) Effects of en-pou5f3 induction at 3-ss and 18-ss on the expression of regulatory genes. For each gene examined, wild-type (−/−) and en-pou5f3 hemizygote (+/−) embryos are shown at the top and bottom, respectively. Up-regulation and down-regulation are marked with solid and open arrowheads, respectively. (A–N, P) Lateral views with anterior to the top and dorsal to the right. (O) Posterior views with anterior to the top. Percentages of embryos with the shown patterns and total embryo numbers are shown in the right-bottom. nc, notochord; nt, neural tube; pnt, posterior-most neural tube; psm, presomitic mesoderm; RA, retinoic acid; sm, somitic mesoderm; tb, tail bud; vm, ventral mesoderm. Scale bars, 200 μm.