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Fig. 5 ap2s1 modulates visually evoked behavior selection and habituation learning (A) Total responsiveness (A) to 6 dark flash (DF) stimuli presented at 150 s ISI by sibling (blue, n = 234) and ap2s1p199 mutant larvae (red, n = 79), two-tailed Mann-Whitney test. (B) Response latency of large angle turns (>95°) by siblings (blue) and ap2s1p199 mutants (red) following DF stimuli in A, two-tailed Mann-Whitney test, ∗∗∗∗p < 0.0001. (C) Relative frequencies of 13 behavioral bout types in response to DFs between siblings (blue) and ap2s1p199 mutants (red). Forward swims and turning bouts are placed roughly in order of increasing vigor, as classified by Marques et al.,53 including Approach Swim (AS), Slow Swim 1 (Slow 1), Slow Swim 2 (Slow 2), Short Capture Swim (SCS), Long Capture Swim (LCS), Burst Swim (BS), J-turn (J-turn), High Angle Turn (HAT), Routine Turn (RT), Spot Avoidance Turn (SAT), O-Bend (O-Bend), Long Latency C-start (LLC), and Short Latency C-start (SLC). (D‒G) Absolute probabilities of DF response behaviors by siblings (blue) and ap2s1p199 mutants (red), focusing on SATs (D, Mann-Whitney U = 7842, two-tailed Mann-Whitney test, ∗∗∗p = 0.0001), LLCs (E, Mann-Whitney U = 10110, two-tailed Mann-Whitney test, p = 0.367), RTs (F, Mann-Whitney U = 6565, two-tailed Mann-Whitney test, ∗∗∗∗p < 0.0001), and O-bends (G, Mann-Whitney U = 570.5, two-tailed Mann-Whitney test, p = 0.921). See also Figure S1 for details. (H) Habituation of large angle turn responses of siblings (blue, n = 214) and ap2s1p199 mutants (red, n = 69) to eight blocks of six identical DF stimuli, presented at 150s ISI for the first block and 15s ISI for subsequent blocks. Habituation assessed by two-way repeated measures ANOVA (Genotype ∗∗p = 0.0043, Time ∗∗∗∗p < 0.0001, Individual ∗∗∗∗p < 0.0001). Data are represented as mean ± SEM in all panels.