Fig. 4

Fig. 4 A target gene of miRNA-7145 was identified by bioinformatics analysis and fluorescence reporting experiments.

A Venn diagram of predicted miRNA-7145 targeted-genes using three different methods. B KEGG enrichment analysis of common targeted-genes predicted by the three different methods. C List of targeted-genes located in the nucleus and associated with myeloid cells. D qRT-PCR showing RNA expression in control embryos injected with miRNA-7145 mimics. The embryos were injected with 200 pg miRNA-7145 mimics at the 1 cell stage, and the embryos were collected at 26 hpf. Relative expression is normalized to β-actin. Data are presented as mean ± SEM. E Expression of EGFP and cuedc2 fusion. All components were injected with 200 pg RNA at the 1 cell stage. Scale bar, 2 mm. *P < 0.05, **P < 0.01, ***P < 0.001.