Fig. 3

Fig. 3 Effect of miRNA-7145 knock-down on hematopoiesis.

Embryos were injected with 10 ng miRNA-7145 MO at the 1 cell stage, and the embryos were collected at 26 hpf and 4 dpf. Agata1a expression, B the cardiac edema phenotype occurs 4 dpf after miRNA MO injection, CRunx1 expression. D–H Detection of myeloid cell marker expression at 26 hpf with whole mount in situ hybridization. Dlyz expression, FPu.1 expression, Gmfap4 expression. E Statistics of lyz-positive cells and H statistics of mfap4 positive cells. I qRT-PCR showing relative RNA expression in control and embryos injected miRNA MO. Relative expression is normalized to β-actin. Data are presented as mean ± SEM. Scale bar is shown in the Figure. *P < 0.05, **P < 0.01, ***P < 0.001.