ZFIN Image: Xu et al., 2024, Fig. 2

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Figure Caption

Fig. 2 Effect of miRNA-7145 overexpression on hematopoiesis.

The embryos were injected with 200 pg miRNA-7145 mimics at the 1 cell stage, and the embryos were collected at 26 hpf. A–E Detection of myeloid cell marker expression at 26 hpf with whole mount in situ hybridization. Alyz expression, Cmfap4 expression, EPU.1 expression. B Statistics of lyz positive cells and D Statistics of mfap4 positive cells. Fgata1a expression, GRunx1 expression at 26 hpf. H qRT-PCR showing relative RNA expression in control and in embryos injected with miRNA mimics. Relative expression is normalized to β-actin. Data are presented as mean ± SEM. Scale bar is shown in the Figure. *P < 0.05, **P < 0.01, ***P < 0.001.

Acknowledgments

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