|
Fig. 2 The metabolic disruptions in zebrafish after chronic exposure to environmental factors. (A) The expression profiles & variable importance in projection (VIP) of metabolites in the HF-exposed zebrafish were significantly disrupted compared to the control, and included a total of 21 pos and neg in females and 12 pos and neg in males. (B) The expression profiles & VIP of metabolites in the HM-exposed zebrafish were significantly disrupted compared to the control, and included a total of 20 pos and neg in females and 30 pos and neg in males. (C) The expression profiles & VIP of metabolites in MC-LR exposed zebrafish were significantly disrupted, compared to control, and included a total of 19 pos and neg in females and 30 pos and neg in males. (D) The expression profiles & VIP of metabolites in the HFMM-exposed zebrafish were significantly disrupted compared to the control, and included a total of 14 pos and neg in females and 30 pos and neg in males. The heatmap with red and green boxes indicated the abundance ratio, and the top 30 metabolites were presented with VIP-value >1 and p-value <0.05 and based on the PLS-DA model.