ZFIN Image: Wang et al., 2024, Fig. EV2

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Figure Caption

Fig. EV2 Impact of ROS on osteoclasts differentiation from PsA961 (NOX4Y512C). (A) Cells were cultured in the presence of M-CSF or M-CSF and RANKL. Visualization of ROS detected by DCFH-DA on Day 6 and Day 11, indicating a higher ROS effect in PsA961-differentiated osteoclasts. Scale bar: 100 μm. (B) The relative fluorescence intensity of ROS was quantified by GraphPad Prism9.0.0. N = 3. Data information: DCFH-DA 2′,7′-dichlorofluorescein diacetate. N = biological replicates. For graph (B), error bars in figure represent mean ± SD (two-way ANOVA with Tukey’s multiple comparisons tests). Source data are available online for this figure.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Mol. Med.