ZFIN Image: El Omar et al., 2024, Fig. 1

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Figure Caption

Fig. 1 Transgene transfer and GFP expression after transduction of human CD34+ and Jurkat cells.

A Human CD34⁺ blood cells from umbilical cord blood and Jurkat cells were transduced by spin inoculation with lentiviral vectors encoding a nuclear variant of the eGFP at multiplicity of infection (MOI) of 30 and 120 and cultured in vitro. After 5 days, cells were analyzed for GFP expression by both flow cytometry and fluorescence microscopy. B Estimates of the efficiency of transduction based on the percentage of GFP⁺ cells. C Percentage of cells expressing CD34 after 5 days of culture. D, E A representative experiment showing GFP expression after transduction of human CD34⁺ or Jurkat cells at the MOI of 30. In (E), results are represented as histograms of GFP fluorescence intensity (x-axis, log scale) versus cell number (y-axis, linear).

Acknowledgments

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