Fig. 4.

Fig. 4.

GABAergic PCs and INs are derived from Ptf1a-expressing neural progenitors. (A-F) Expression of Pvalb7 and GFP in 5 dpf TgBAC(gad1b:LOXP-DsRed-LOXP-GFP) larvae that were treated with DMSO (control, n=5; A-C) or endoxifen (n=5; D-F) at 2 dpf. (G-R) Expression of Pvalb7 and GFP in 5 dpf TgBAC(ptf1a:Gal4-VP16); Tg(UAS-hsp70l:mCherry-T2A-CreERT2); TgBAC(gad1b:LOXP-DsRed-LOXP-GFP) larvae that were treated with DMSO (n=5; G-L) or endoxifen (n=5; M-R) at 2 dpf. The larvae were stained with anti-Pvalb7 (cyan), anti-RFP (magenta) and anti-GFP (green) antibodies. Dorsal views with anterior to the left. The cerebellum region (Ce) is surrounded by a dotted line. (J-L,P-R) Higher magnification views of the boxed areas in G-I,M-O. Arrows and arrowheads indicate Pvalb7⁺ GFP⁺ cells (PCs) and Pvalb7⁻ GFP⁺ cells (INs), respectively. Scale bars: 50 μm (in A, for A-F; in G, for G-I,M-O); 10 μm (in J, for J-L,P-R). (S-U) Total number of GFP⁺ cells (S), Pvalb7⁺ GFP⁺ cells (T) and Pvalb7⁻ GFP⁺ cells (U) in the cerebellum of larvae treated with DMSO or endoxifen. *P<0.05, ***P<0.001, ****P<0.0001 (two-way ANOVA followed by Bonferroni multiple comparisons). Data are shown as mean±s.e.m. with individual values indicated.