Fig. 5

Fig. 5

PAX1 ectopic expression leads to impaired definitive endoderm differentiation by inhibiting Wnt signaling. A Flow cytometry analysis of wild-type (WT) control and PAX1 overexpressed (OE) hESC-derived definitive endoderm cells stained for SOX17 (Alexa Fluor 488) demonstrates decreasing of SOX17⁺ population when PAX1 was ectopically expressed for a representative differentiation (n = 3 independent differentiations). B Heatmap representation of RNA-seq analysis of definitive endoderm cells derived from WT and PAX1 OE hESCs (n = 2; log2 fold change ≤ − 1, ≥1; p-value < 0.05). Differentially expressed known Wnt targets, endoderm markers and signaling molecules were listed above. C qRT-PCR analyses of endoderm marker genes in hESC-derived definitive endoderm cells. D Genome tracks of TCF7, TCF7L1, TCF7L2 and LEF1 ChIP-seq reads analyzed from datasets (GSE182842) on definitive endoderm marker genes in human pluripotent stem cell-derived definitive endoderm cells. E qRT-PCR analyses of known Wnt target genes in hESC-derived definitive endoderm cells