Fig. 1

Fig. 1

PAX1 plays a negative role in canonical Wnt signaling pathway. A-B TopFlash luciferase reporter assays in HEK293FT cells transfected with an empty vector control or a PAX1 expression plasmid, with or without treatment of GSK3 inhibitor CHIR99021 (CHIR) (A) or LiCl (B). RLA: relative luciferase activity. C-D TopFlash luciferase reporter assays in HEK293FT cells with transfection of an increasing dose of HA-tagged PAX1 and activated Wnt signaling by co-transfection of flag-tagged β-catenin (β-Cat) (C) or treatment with CHIR99021 (D). E-F Quantitative real-time PCR (qRT-PCR) analyses of Wnt target genes performed in HEK293FT cells with or without CHIR99021 treatment. G Expression of Wnt target genes by in situ hybridization in WT (wild type) control, pax1 mRNA or morpholino (MO) injected zebrafish embryos. The frequency of embryos with the indicated patterns is shown in the bottom right corner of each group. Bar graphs and error bars represent mean ± standard deviation (SD) of at least three biologically independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001. This is the same in the following figures unless specifically indicated