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Fig. 8 Neutralization of IL-16 contributes to host control of Mycobacterium infections. A Schematic diagram of GAPBAα/Pro-IL16/HDAC3 complex regulates Rev-erbα expression and IL-16 regulates the maturation of phagolysosomes through CD4. B Schematic diagram of neutralization of IL-16 in vivo. 6-8 weeks of age mice were intraperitoneally injected with 200 μg anti-IL-16 neutralizing mAb 2 h prior to infection. Mice were challenged with 3×106 CFU of H37Ra in 100 μL PBS via the intravenous route, followed by intraperitoneal injection 3 times weekly with 200 μg anti-IL-16 neutralizing mAb. Control mice received 200 μg isotype-matched mouse IgG2a(κ). The mice were sacrificed 4 weeks post-infection (n = 3 to 5). C IL-16 level in BALF by ELISA (n = 3 to 5). D H37Ra load in lung (n = 3 to 5). E TNF, IL-6, IL-10 and TGF-β levels in BALF measured by ELISA (n = 3 to 5). *p<0.05 compared to media, Student's t-test. The graph shown is representative of 2 independent experiments.