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Fig. 5 Vc-responsive BMP activation in EBs arrests intestinal stem cell growth non-cell autonomously (A) Posterior midguts of Su(H)ts/+ adult flies uninfected or infected with C6706 for 36 h. Hoechst labels DNA (blue); Delta marks ISCs (yellow); GFP labels Su(H)+ EBs (green); pMAD labels cells with BMP activation (magenta). Arrows and dashed box: pMAD+ GFP+ EBs. Scale bars in dashed boxes: 5 μm. Scale bars in images: 15 μm. (B) Proportion of intestinal stem cells or EBs that are pMAD+ in Su(H)ts/+ intestines. (C–E) Proportions of all cells that are (C) progenitors, (D) ISCs, or (E) EBs in the intestines with EB-specific BMP inactivation (Su(H)ts>shnRNAi). Each dot represents a measurement from a single fly gut. p values are calculated using unpaired Student’s t tests.