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Fig. 4 Rab11bb knockdown suppressed the capacity of M-cells axon regeneration. (A) Schematic diagram of working principle of primary miR-shRNA gene silencing system (RISC: RNA-induced silencing complex). (B) The position of shRNA1–3 in open reading frame (ORF) of rab11bb (UTR: untranslated region). (C) Schematic diagram of microinjection using the miR-shRNA system. (D) qRT-PCR analysis of rab11bb knockdown efficiency. The total RNA of each group (n = 30) was obtained from three independent experiments (shRNA1: 71.7 ± 3.0%; shRNA2: 84.0 ± 3.4%; shRNA3: 59.2 ± 2.7%). (E) Timeline of time points of electroporation, axotomy and imaging. (F) Representative diagram of confocal imaging of M-cells axon regeneration. Asterisk, ablation site; Scale bar, 50 μm (Ctrl: control; KD: knockdown). (G) Statistical quantitative diagram of axon regeneration (control: 280.6 ± 34.48 μm, n = 11; Rab11bb knockdown: 131.0 ± 18.63 μm, n = 13). Assessed by unpaired, two-tailed Student's t-test. *** p < 0.001.