Fig. 10 Hecubine exhibited an antioxidative effect in LPS-stimulated zebrafish larvae. Zebrafish larvae were treated as described above. (a) ROS levels were measured by image analysis and fluorescence microscopy; Kruskal-Wallis test of One-way ANOVA (n = 5). (b) Fluorescence intensity was quantified using ImageJ. The protein levels of Nrf2 (n = 4), HO-1 (n = 3) (c), and TREM2 (n = 4) (d) were assessed by Western Blot. The data are the mean ± SEM of at least three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. LPS-treated group; ###p < 0.001 vs. control; One-way ANOVA with Dunnett’s multiple comparisons test.
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