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Fig. 9 Hecubine modulated locomotor deficits and exhibited anti-inflammatory activity in LPS-stimulated zebrafish larvae. (a) Schematic of compound administration in zebrafish. (b) Toxicity of Hecubine in the zebrafish model (n = 12 zebrafish per group). (c) Representative patterns of behavioral locomotion in zebrafish larvae and quantitative analysis of zebrafish locomotion in the control and treated groups (n = 9∼10 zebrafish per group). (d) Production of NO measured by Griess assay (n = 3). (e–g) Protein expression of Ibal (n = 3) (e), iNOS (Kruskal-Wallis test of One-way ANOVA, n = 4) (f), and IL-1β (n = 5) (g) was quantified by Western Blot. (h) The mRNA expression levels of TNF-α, IL-1β, and IL-6 were analyzed by qPCR, n ≥ 4. The data are mean ± SEM. *p < 0.05 and **p < 0.01 vs. LPS-treated group; ###p < 0.001 vs. control; One-way ANOVA with Dunnett’s multiple comparisons test.