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Fig. 8 Blockade of Nrf2 abolished the anti-inflammatory effect of Hecubine. BV2 cells were treated with Hecubine in the presence or absence of LPS after transient transfection of Nrf2 siRNA and control siRNA. The protein levels of Nrf2 (a), HO-1 (b), TLR4, and MyD88 (c) were determined by Western blotting (n = 3). The production of TNF-α, IL-6, and NO (d) was measured via Griess reagent and an ELISA assay, respectively (n = 3). The mRNA levels of iNOS, TNF-α (e), IL-6, and IL-1β (f) were detected by qPCR analysis (n = 4). The data from the experiments are expressed as the mean ± SD of three independent experiments. *p < 0.05 vs. LPS-treated group; #p < 0.05 vs. control; One-way ANOVA with Dunnett’s multiple comparisons test.