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Fig. 2 Hecubine directly binds to TREM2. (a) The interaction of Hecubine with the TREM2 protein in molecular docking analysis. (b) The CETSA assay determined the thermal stabilization of TREM2 interaction with Hecubine at a series of temperatures from 37 °C to 69 °C under a short drug exposure (1 h). (c) Western blot analysis of TREM2 knockdown in BV2 cells. Cells were transfected with negative control shRNA or TREM2 shRNA. n = 3; two-way ANOVA with Dunnett’s multiple comparisons test. (d) qPCR analysis of TNF-α and IL-1β in BV2 cells transfected with TREM2 shRNA. n = 3; two-way ANOVA with Dunnett’s multiple comparisons test. The data are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. LPS-treated group; ns, not significant.