ZFIN Image: Liu et al., 2024, FIGURE 5

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FIGURE 5

Sema3E binds to Plexin B3 in vitro. The binding assay was performed using type 3 Semaphorins tagged with alkaline phosphatase (AP-Sema3) according to He et al. (2019). (A) Supernatant containing AP-tagged Sema3E was applied to HEK293 cells transfected with pAG3 empty vector. No obvious binding of Sema3E to the cells was detected. (B) AP-tagged Sema3E binds to cells expressing Nrp1a. (C,D) Sema3E or Sema3D binds to Plexin B3. (E,F) Sema3E or Sema3D binds to the cells co-transfected with Plexin B3 and Nrp1a.

Acknowledgments

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