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Fig. 3 The activity of ADP ribosyl cyclase is implicated in myeloid differentiation and bactericidal activity induced by ZGSII, BA, and DMA. (A) Effects of ZGSⅡ (10 μM), BA (10 μM), DMA (10 μM), or C3G (40 μM) on CD11b expression after 5 days of treatments. (B) The histogram shows the percentage of CD11b+ cells for each group. (C) Giemsa-Wright staining of NB4 cells treated with ZGSⅡ, DMA, BA, or C3G for 5 days. Yellow arrows represent differentiated NB4 cells. Bars = 10 µm. (D) Bactericidal activity evaluation. The cells were treated with ZGSⅡ, BA, DMA, or C3G for 5 days, followed by a bacterial killing assay. (E, F) The histograms display the percentage of differentiated NB4 cells (E) and the number of killed bacteria (F) for each group. The data are presented as the mean ± SD of three independent experiments. *p < 0.05; * *p < 0.01; * **p < 0.001 versus the corresponding control groups (one-way analysis of variance).