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Fig. 2 TPhP induced oxidative stress and activated Nrf2/Keap1/HO-1 signaling pathway in zebrafish larval. (A) ROS production induced by TPhP in zebrafish at 72 hpf. (B-C) The expression of HO-1 and Keap1 of zebrafish (96 hpf) exposed to TPhP. (D-E) The expression of HO-1 and Keap1 of zebrafish (7 dpf) exposed to TPhP. (F-G) The transcriptional expression of related genes in the Keap1/Nrf2 pathway of zebrafish (96 hpf and 7 dpf) exposed to TPhP. The treatment groups were composed of three replicates, each containing 25 larvae. Values represent mean ± SEM. In comparison with the control group, * p < 0.05, * * p < 0.01, * ** p < 0.001 (one-way ANOVA and LSD test was employed).