Figure 5.

Figure 5. VLZ rescues bone marrow and splenic hematopoiesis after radiation injury.

(a, b) H&E staining shows the megakaryocytes in BM and spleen after mice were treated with normal saline, thrombopoietin (TPO) (3000 U/kg), and VLZ (2.5, 5, and 10 mg/kg) for 12 days. Ten microscopy fields per sample were counted. The circles mark the megakaryocytes. The histogram shows the number of megakaryocytes in the BM and spleen in each group. (c) The examination of the expression of c-Kit and CD41 in each group by flow cytometry after receiving therapy for 12 days. The histogram represents the percentage of c-Kit⁺/CD41^-, c-Kit⁺/CD41⁺, and c-Kit^-/CD41⁺ cells in each group. (d, e) Flow cytometry analysis indicates the expression of CD41 and CD61 in BM and spleen cells after receiving therapy for 12 days. The histogram represents the percentage of CD41⁺/CD61⁺ cells in each group. (f, g) Flow cytometry analysis indicates the cell ploidy of BM and spleen cells of each group. The histogram represents the cell ploidy in the BM and spleen cells of each group. All data represent the mean ± SD of three independent experiments. *p≤0.05, **p≤0.01, and ***p≤0.001, vs the model group.