Figure 1

Figure 1

ATP and adenosine (Ado) content in the whole liver of adult zebrafish and HepG2 culture supernatant after ethanol (EtOH) exposure. (a,b) Gross appearance of zebrafish and liver (white dotted line) without (a) or with (b) 0.1% EtOH exposure for 4 weeks. Scale bars 5 mm (top panel) and 2 mm (bottom panel). (c,d) Representative hematoxylin and eosin (HE) staining of the liver sections from adult zebrafish without (c) or with (d) 0.1% EtOH exposure. Lipid droplets in the EtOH group are indicated with black arrows. Scale bar 50 µm (lower magnification) and 40 μm (higher magnification). (e,f) ATP (e) and Ado (f) content in the whole liver tissue of adult zebrafish without or with 0.1% EtOH exposure (n = 10, each group). (g,h) ATP (g) and Ado (h) content in HepG2 culture supernatant with or without EtOH (60 or 300 mL/L) addition (n = 5, each group). Data are mean ± SEM. P values were calculated using the Student’s t-test (e,f) or one-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test (g,h). *P < 0.05; **P < 0.01; ***P < 0.001 vs. control. n.s. not significant.