Figure 3

Figure 3

Role of Hs791 and Hs2160 on transcriptional expression control. (A) Luciferase assay performed in HEK293 cells transfected with empty pGL3-promoter vector plasmid (EV, empty vector) or pGL3-promoter vector plasmid containing the wild type (wild-type PQS, black bars) or mutated (mutated PQS, grey bars) sequence of Hs791 and Hs2160 upstream the basal promoter SV40. Each bar represents the luciferase activity normalized to β-galactosidase activity and relativized to empty pGL3-promoter vector plasmid. Bars represent the mean of three independent experiments ± SEM. **P < 0.01, t test. (B) Effect of chemical stabilization of G4 on TCOF1 endogenous transcriptional expression in HEK293 cells. Pyridostatin (PDS), a well-known G4 stabilizer, was added to cells at the indicated concentrations for 15 h. RNA was isolated and TCOF1 expression was analyzed by RT-qPCR. Values are relativized to the non-incubated control (0 PDS). Bars represent the average of 3 independent experiments ± standard error of the mean. NS not significant, *P < 0.05, t test.