Survival of M-AB embryos was hardly affected by microinjections. (A) Survival of chordin MO-injected M-AB and *AB embryos (n = 5). (B) Approximately 80% of chordin MO-injected embryos showed chordin-deficient phenotypes. The numbers in the color bars indicate the number of samples. (C) Survival of M-AB and *AB embryos injected with sqstm1 sgRNA and/or Cas9 protein (n = 4). (D) Survival of M-AB and *AB embryos/larvae injected with ef1α:EGFP plasmid and tol2 mRNA (n = 9). Plots connected by lines indicate the same experimental group. (E) Percentages of fish (30 dpf) that harbor GFP-transgene detectable by PCR (*AB: n = 3, M-AB: n = 7). Error bars indicate the standard deviation.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Sci. Rep.
Your Input Welcome
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if
additional information is required.
Oops. Something went wrong. Please try again later.