ZFIN Image: Lv et al., 2024, Figure 5

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Figure 5

prpf31^−/−zebrafish exhibit severe aberrant alternative splicing of mitosis-related genes.A, DASEs corresponding to three representative GOBP terms, that is chromosome organization (GO: 0051276), regulation of cell cycle (GO: 0051726), and microtubule-based process (GO: 0007017) were visualized by heatmaps of normalized PSI. B, the DASEs of the representative genes were confirmed by SqRT-PCR. Data were shown as mean ± SD of three independent experiments (n = 3); unpaired two-tailed t test; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001. DASE, differential alternative splicing event; PRPF, pre-mRNA processing factor; PSI, percent spliced in; SqRT-PCR, semiquantitative reverse transcription PCR. GOBP, Gene Ontology biological processes.

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