Cell proliferation is higher in the ears of young zebrafish. (a) Representative confocal images of inner ear epithelia from young (top row) and old (bottom row) adult zebrafish. The scale bar in the top left image applies to all images. Epithelia are outlined with white dotted lines. (b) Quantification of BrdU + cells in each epithelium in young (green bars) and old (gray bars) adult zebrafish. There is a significant effect of age on BrdU + cell count (2-way ANOVA, F1,80 = 78.32, p < 0.0001). All Bonferroni-corrected pairwise comparisons are significant **p < 0.01, ****p < 0.0001. Data are presented as mean ± 1 s.d. and dots represent individual fish. N = 14–17 epithelia from young fish, n = 13–14 epithelia from old fish.
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