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Fig. 2 Key residues of mTRIM71-NHL for RNA-binding. (a) EMSA results of 11 nt Trincr1 RNA bound to wild-type mTRIM71-NHL or mutants (WT, R612A, R642A, K659A, D660A, Y689D, Y689A, R707A, R738A, R783A and R801A). Trincr1 RNA was similarly shifted to a constant position by wild-type and D660A and R707A mutants. Other mutants showed varying degrees of defects in RNA-binding properties. (b) FP results quantified the binding affinities of wild-type mTRIM71-NHL or mutants to the 11 nt Trincr1 RNA. KD values were shown and error bars were SEM. Six technical replicates (n = 6) were measured for the binding reaction of the wild-type protein, and three replicates (n = 3) were measured for each of the mutants.