Fig. 7

Fig. 7

Gross histology and ORO stain. Toluidine blue-stained retinal sections of 1-year-old wild-type and homozygous Elovl4b KO fish. We observed no significant differences in the gross morphology and lamination of the retinas from both groups. Images were taken at 4× (A) and 40× (B) magnification. Cryosections of 1-year-old age-matched male wild-type and homozygous Elovl4b KO zebrafish retinas, treated with ORO to stain lipids (red) and counterstained with hematoxylin (blue) (C). The red arrows point to lipid-rich ORO-stained puncta localizing in the basal RPE of homozygous Elovl4b KO mutants. Images were taken at 40× with a Zeiss AxioScan slide scanner. GCL, retinal ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; IS, inner segment; ONL, outer nuclear layer; OPL, outer plexiform layer; OS, outer segment; RPE, retinal pigment epithelium.