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Fig. 2

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Publication: Nwagbo et al., 2024 - Elovl4b Knockout Zebrafish as a Model for Ocular Very-Long-Chain Polyunsaturated Fatty Acid Deficiency
All Figures: Figures for Nwagbo et al., 2024

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Fig. 2

Sequencing and PCR analysis. A: Gel electrophoresis showing wild-type, heterozygous, and homozygous Elovl4b KO zebrafish. DNA was extracted from caudal fin clips and amplified using PCR. The PCR product was subsequently run on 2% agarose gel at 110 V. This image was captured using an iBright CL750 imaging system (Invitrogen, Carlsbad, CA). B: Nucleotide sequence alignment between wild-type and homozygous Elovl4b mutant fish shows that a 56 bp deletion mutation at exon 2 of zebrafish Elolv4b leads to a premature termination codon at position 5 of the protein-coding sequence. The start codons are highlighted in yellow, and the premature termination codon is highlighted in green.

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