Figure 2

Figure 2

Amoxicillin treatment has limited impact on zebrafish liver function. (A–D) Quantification of triglyceride, total cholesterol, ALT activity, and AST activity in 5 dpf larvae after amoxicillin treatment from 0.5 to 5 dpf. Three independent experiments. Mean + s.e.m. In ANOVA analysis, ns—not significant. (E) Representative Oil red O [3] staining of larvae at 7 dpf. Larvae were categorized as having none, mild, or severe hepatic steatosis. Arrows point to the liver area. (F) Quantification of hepatic steatosis by ORO staining in 7 dpf larvae after amoxicillin treatment. The numbers of larvae analyzed in each group are indicated. In Chi-square tests, ns—not significant. (G) WISH revealed the expression of cp and abcb11b at 7 dpf after amoxicillin treatment. Numbers indicate the ratio of representative staining observed. (H) H&E staining of liver tissues at 7 dpf after amoxicillin treatment from 0.5 to 5 dpf. Scale bars, (E) 50 µm, (G) 100 µm, (H) 5 µm. dpf, days post-fertilization; ALT, alanine aminotransferase; AST, aspartate aminotransferase.