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Fig. 2 Effects of verapamil on cardiac phenotype and heart rate of zebrafish embryos A. 52 hpf zebrafish embryos were exposed to various doses of verapamil (0–400 μM) for 30 min. The phenotypes were classified as normal, moderate and severe according to the area of the pericardial sac and venous congestion. The yellow and red-dotted lines indicate the area of the pericardial sac and venous congestion, respectively, scale bar = 200 μm. B. Statistics of phenotypic proportions of zebrafish embryos exposed to different doses of verapamil (0–400 μM) for 30 min, n = 30, the experiment was replicated three times. C. Statistics of heart rate of zebrafish embryos exposed to different doses of verapamil (0–400 μM) for 30 min, n = 30, compared with those of the 0 μM treated group (DMSO group).