Figure 5

Figure 5

Area of NT2/D1/-R and NCCIT/-R tumor xenograft in AB zebrafish embryos exposed to dinaciclib alone or combined with CP. (a) Tumor areas at 120 hpf (T3—end of treatment) of drug-treated and vehicle-treated embryos were measured using Zen 2.3 Black software from ZEISS. Results are reported as area (µm²) ± SEM. * p < 0.0001, § p < 0.01, ° p < 0.05 vs. vehicle or single drug as indicated in Table 3. (b) Representative, lateral-view pictures of Tg (kdrl:EGFP) untreated and treated embryos at 120 hpf xenografted with NT2/D1 cells. Cells were labeled with a red fluorescent lipophilic dye, while the embryo endothelium was labeled with a green fluorescent protein reporter driven by the kdrl promoter. Images were acquired using a Zeiss LSM 900 confocal microscope at 10× magnification.