IMAGE

Fig 4

ID
ZDB-IMAGE-240310-4
Source
Figures for Ruan et al., 2024
Image
Figure Caption

Fig 4 Fancl interacts with Tp53 in HEK293T cells.

(A) The interaction of Fancl with Tp53 in HEK293T cells as revealed by the Co-IP assay. Myc-tagged Fancl and Flag-tagged Tp53 were transfected into HEK293T cells, then anti-Flag antibody-conjugated agarose beads were used for immune-precipitation. (B and C) Domain mapping revealed that the DBD domain of Tp53 is required for their interaction. TAD, transactivation domain. DBD, DNA binding domain. TMD, tetramerization domain. (D and E) Domain mapping revealed that the multiple domains, WDR and CTD, of Fancl are required for their interaction. WDR, WD-repeat domain. ULD2, UBC-like domain 2. ULD3, UBC-like domain 3. CTD, C-terminal domain. IP, immunoprecipitation. IB, immunoblotting. TCL, total cell lysate.

Acknowledgments
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