Fig 3

Fig 3

cry1a but not per2 expression is induced upon a pulse of white light at CT21 (A-G) Expression of per2 (A-D) or cry1a (E-G) at CT22 in 7 days old larvae. Before fixation for in situ at CT 22 the larvae were treated exactly like in the experiment described in Fig 2D, meaning that they were not depigmented before the light pulse which was administered from CT21 and CT22 (CT 22 pulse). In parallel, larvae from the same litter were maintained in the dark and fixed at CT22 (CT 22 dark). (A-D) The white ellipse identifies the pineal gland where no expression is observed. ctl DARK: n = 3, ctl PULSE n = 7, lak DARK: n = 4, lak PULSE: n = 7. Scale bar: 50 μm. (E-G) Three different expression patterns can be identified with the cry1a probe, The grey ellipse surrounds the pineal which expresses cry1a in the ‘mild’ and ‘high’ patterns, the two dark ellipses in G surrounds the habenulae which express cry1a in the ‘high’ pattern. Tel = Telencephalon, Tec = Optic tectum. Scale bar: 200 μm. (H) Countings of the repartition of Dark and Pulsed 7 days old larvae at CT22 stained with the cry1a probe, ctl DARK: n = 10, ctl PULSE n = 4, lak DARK: n = 5, lak PULSE: n = 7. Ctl DARK versus Ctl PULSE: p = 0.0010, lak DARK versus lak PULSE: p>0.9999, ctl DARK versus lak DARK: p>0.9999, ctl PULSE versus lak PULSE: p = 0.0182 using Fisher test.