FIGURE 6

FIGURE 6

Maternal vgll4a is required for plasma membrane localization of the E-cadherin/β-catenin complex. (A,I) Schematic representation of the different imaging strategies. (B-G; J-O) Confocal images of F-actin (B, E, J, M), β-catenin (C, F, K, N) and E-cadherin (D, G, L, O) distribution in EVL (B–G) and DEL (J–O) cells in wt and MZvgll4a embryos at sphere stage. Embryos were counterstained with Hoechst (nuclei, green). (H,P) The graphs depict the fluorescent signal intensity (in arbitrary units, a. u) for F-actin, β-catenin and E-cadherin in EVL (H) or DEL (P) cells of wt and MZvgll4a embryos. Mann Whitney test. (H) β-catenin, p = 0.0001, E-cadherin; p = 0.0425. (P) F-actin, p = 0.3154; β-catenin, p = 0.0006 and E-cadherin, p = 0.1599. Ns, not significant, *p < 0.05; ***p < 0.001; ****p < 0.0001. Scale bar, 10 µm.