ZFIN Image: Cadiz et al., 2023, Fig. 5.

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Figure Caption

Fig. 5.

Expression of shha in regenerating gill filaments. (A) Demonstration by RT-qPCR of relative mRNA expression of shha in regenerating gills of zebrafish 10 days after the resection procedure (squares), compared with expression in gills from intact animals (circles). Expression was observed under control conditions, but relative abundance increased during regeneration. Data were normalized to the mRNA abundance of the reference gene, ef1a, and analyzed using the Mann–Whitney U-test (two-tailed). The mean±s.e.m. significantly different from control is indicated by asterisks (U=0, P<0.01; N=8 for each group). (B,C) In situ hybridization demonstrated expression of shha along the length of the filaments (region between arrowheads) in (B) regenerating filaments and in (C) unresected controls, in addition to the increased expression in regenerating tissue (arrow) in B. Scale bar: 100 µm and applies to both panels.

Acknowledgments

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