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Fig. 1 FeSP neurons express scg2a dependent on ovarian estrogen. A) Integrative genomics viewer image of RNA-seq reads from FeSP neurons in the PMm/PMg of intact, sham-operated (sham), and ovariectomized (OVX) females (n = 3 for each treatment) for XLOC_005021 (scg2a). B) Phylogenetic tree showing the relationship of medaka Scg2a to other known granins. The number at each node indicates bootstrap values for 1,000 replicates. Scale bar represents 0.1 substitutions per site. For species names and GenBank accession numbers, see Table S2. CHGA, chromogranin A; CHGB, chromogranin B. C) Levels of scg2a expression in FeSP neurons of females that were sham-operated (sham) or ovariectomized and treated with vehicle only (OVX), estradiol-17β (OVX + E2), or 11-ketotestosterone (OVX + KT) as determined by real-time PCR (n = 6 for all groups except OVX + KT, where n = 5). Mean value of the sham group was arbitrarily set to 1. D and E) Representative micrographs showing the expression of scg2a in FeSP neurons (expressing npba) (D) and it- and vt-expressing neurons (E) in the PMm/PMg. Left and middle panels show images of scg2a (magenta) and npba/it/vt (green) expression, respectively, in the same section; right panels show the merged images with nuclear counterstaining (blue). Arrowheads indicate representative neurons coexpressing scg2a and npba/it/vt. Scale bars represent 50 μm. Statistical differences were assessed by Bonferroni's post hoc test (C). **P < 0.01; ***P < 0.001.