ZFIN Image: Zhao et al., 2023, Fig. 1

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Figure Caption

Fig. 1 Analysis of morphant phenotype and TAA-induced embryonic liver lesions caused by Yap knockdown. A Validation of Yap knockdown by RT-PCR and Western blot analysis. Total RNA and proteins were extracted from 24 hpf zebrafish embryos in MO (Yap) and MO (Ctrl) (n = 10). B Validation of Yap knockdown by qPCR. C Morphant observation of 48 and 72 hpf embryos injected with MO (Yap) (5 ng/embryo), MO (Ctrl) (5 ng/embryo), and non-injected normal embryos. D Nile red-stained sections of embryos with the treatment of 0.025% TAA from 72 hpf to 7 dpf. E Qualifications of liver size in each group, n = 5. F Qualifications of Nile red staining by relative red intensity by ImageJ (n = 5). Data are shown as means ± SEM. *p < 0.05. G Histological evaluations of zebrafish liver by hematoxylin and eosin (H&E) with the treatment of 0.025% TAA from 72 hpf to 7 dpf

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Commun. Signal.