Fig. 4.

Fig. 4.

Affinities between p53TAD peptides and MDM2 from D. rerio. a) The overlapping peptides tiling a 51-residue stretch of p53TAD_D.rerio. To facilitate comparison, the numbering starts from the conserved motif and the residues of human p53TAD, F₁₉xxxW₂₃xxL₂₆. With that in mind, the p53TAD^10–75 in fact contains the whole TAD region of D. rerio and corresponds to p53TAD^full-length. K_D values for the peptide fragments were determined by the fluorescence polarization assay. Distal residues and Ile₂₈Ser₂₉Ile₃₀ contribute to the affinity for MDM2. Both conserved motifs are in bold. The zebrafish silhouette was obtained from phylopic.org and is under CC0 1.0 DEED licence (https://creativecommons.org/publicdomain/zero/1.0/). b) ColabFold prediction of the interaction between D. rerio MDM2 and p53TAD^10–75. The MDM2 is in gray, and the p53TAD^10–75 is colored according to the pIDDT, where blue indicates highest and red lowest prediction confidence (pIDDT < 50).