ZFIN Image: Shi et al., 2023, Fig. 5

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Figure Caption

Fig. 5

FGF19 influences ANXA2 expression to accelerate angiogenesis. A: ANXA2 expression in HUVECs with the increasing doses of FGF19. B: The interference efficiency of shANXA2 was assessed by western blotting in HUVECs. C, D: Tube formation assays (top) and Transwell migration assays (bottom) were performed to measure tube formation and migration of HUVECs transfected with shNC or shANXA2. E: Western blotting was used to detect ANXA2 expression with the treatment of FGF19 or shANXA2. F: Tube formation assays (top) and Transwell migration assays (bottom) were performed to measure tube formation and migration of HUVECs. G, H: The relative tube length and migrated HUVECs were quantified. I: HUVECs transfected with shANXA2 or shNC and pretreated with FGF19 were mixed with Matrigel for subcutaneous injection. Top: Gross observation of angiogenesis in Matrigel plugs. Bottom: H&E staining was performed to observe blood vessel formation in different groups. Data represent the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001

Acknowledgments

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