Fig. 2

Fig. 2

FGF19 regulates NPC cell malignant behaviours. A: Western blot analysis of FGF19 expression in NPC cell lines (CNE1, CNE2, 5-8F, 6-10B, C666-1) and the immortalized normal nasopharyngeal epithelial cell line NP69. B: ELISA was used to detect FGF19 level in culture medium(CM) of different cells. The column showed FGF19 concentration in CM from NPC cells relative to CM from NP69. C: The interference efficiency of shFGF19 was assessed by western blotting in CNE2 and CNE1 cells. D: CCK8 assay was used to determine cell proliferation after transfection with shNC or shFGF19 in CNE2 and CNE1 cells. E, F: Colony formation assay was performed in shNC or shFGF19 cells. We showed the representative images and the quantification analysis. G, H: Transwell assay was used to determine cell migration in shNC or shFGF19 cells. We showed the representative images and the quantification analysis. I, J: Wound healing assay was performed in shNC or shFGF19 cells. Representative images of cell migration were captured at 0 and 48 h with a microscope. The relative migrated width was calculated by the wound width/the distance measured at 0 h. The histogram showed the relative distance of wound. K: Tg (fli1a: EGFP) transgenic zebrafish were used to evaluate cell metastasis. shNC or shFGF19 CNE2 cells were stained with Dil and injected into the perivitelline cavity of zebrafish at 48 hpf. The migration of tumour cells was evaluated 2 days postinjection. The arrow represented the disseminated foci. We observed the disseminated foci from primary sites under a fluorescence microscope. Data are presented as the mean ± SD of three independent assessments. *P < 0.05, **P < 0.01, ***P < 0.001