Figure 8

Figure 8

(A) Average inter-fish distance, (B) average shoal area, (C) average nearest neighbor distance, and (D) average farthest neighbor distance of zebrafish after being exposed to tacrolimus at two different concentrations: 1 ppb (red) and 100 ppb (blue) compared to control (black). The data are expressed as the median with an interquartile range. The statistical analyses were conducted using the Kruskal–Wallis test, followed by Dunn’s multiple comparisons test. The statistical comparisons were performed between the control and each treated group (shoal size = 3 fishes; n control & 1 ppb = 27, n 100 ppb = 30; ns = not significant, * p < 0.05, ** p < 0.01, *** p < 0.001).