ZFIN Image: Zhou et al., 2024, Figure 5

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Figure 5

Metformin downregulates H3K18 lactylation. (a) Lactate levels in zebrafish larvae significantly decreased after treatment with metformin. (b–d) Q-PCR analysis showed that the lactate metabolism-related gene hdac3 was significantly upregulated and that the expression levels of pkma and gapdh were significantly downregulated in zebrafish larvae after metformin treatment. (e–g) Western blotting results showed that the levels of histone lactylation and histone lactylation (H3K18) were significantly reduced in zebrafish larvae after metformin treatment. (h) Lactate levels in murine macrophages significantly decreased after treatment with metformin. (i–k) Q-PCR analysis showed that the lactate metabolism-related gene hdac3 was significantly upregulated and that the expression levels of pkma and gapdh were significantly reduced in murine macrophages after metformin treatment. (*** p < 0.001, ** p < 0.01, * p < 0.05).

Acknowledgments

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