Figure 3

Figure 3

Metformin can reduce reactive oxygen species levels in zebrafish and cells (a,b). The results of fluorescence staining showed that metformin downregulated the level of ROS in zebrafish and that H₂O₂ upregulated the level of ROS (scale bar: 200 μm). The addition of metformin alleviated the effect of H₂O₂ on reactive oxygen species in zebrafish (n = 25). (c–e) Q-PCR results showing that metformin treatment reduced the upregulation of H₂O₂-induced expression of duox, sod1, and cat in zebrafish. (f,g) Fluorescence staining results showing that metformin downregulated ROS and that H₂O₂ upregulated ROS in murine macrophages. The addition of metformin alleviated the effect of H₂O₂ on reactive oxygen species in murine macrophages. (h–j) Q-PCR results showing that metformin treatment reduced the upregulation of H₂O₂-induced expression of duox, sod1, and cat in murine macrophages. (**** p  <  0.0001, *** p  <  0.001, ** p  <  0.01, * p  <  0.05, “ns”, no statistical difference).