ZFIN Image: Oudhoff et al., 2024, Fig. 6

Image

Figure Caption

Fig. 6 Comparison between smyhc1 and smyhc1:LY-Tomato expression in uninjured and regenerating muscles.

Transversal sections of smyhc1:LY-Tomato transgenic fish, stained by in-situ hybridization (purple), Tnnt2 antibody (red) and DAPI (blue). N = 3 fish per group. The frames depict the areas that are enlarged in the images to the right. Scale bar indicates 200 μm. a, b In uninjured fish, Tnnt2 positive muscle is outlined with a dashed line. The smyhc1 probe (a) labels the entire Tnnt2 positive area, corresponding to the slow muscle. The LY-Tomato probe (b) detects only the superficial-most layer of the slow muscle. c, d At 7 dpci, the smyhc1 probe (c) displays a scattered labeling in the cryoinjured side, but no Tnnt2 staining. A similar expression is detected for the LY-Tomato probe (d). Scale bar indicates 200 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ NPJ Regen Med