ZFIN Image: Ramesh et al., 2024, FIGURE 4

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Figure Caption

FIGURE 4

Clustal-based phylogenetic tree of mice (m) and zebrafish (z) Hes1/5/7 orthologs. Amino acid sequences of mHes7 (NP_149030.2), mHes5 (NP_034549.1), mHes1 (P35428) zher1 (Q90463), zher7 (Q9I9K1), zher11 (Q6W4T8), zher2 (Q90464), zher4 (Q90466), zher12 (Q6TA36), zher15 (Q7T3J0), and zher6 (Q6PBX3) were first aligned in the FASTA format using MUltiple Sequence Comparison by Log- Expectation (MUSCLE). The FASTA alignment file was input into the EMBL-EBI Simple Phylogeny tool. The tree was generated in a Clustal format with distance corrections and a UPGMA (Unweighted Pair Group Method with Arithmetic Mean) clustering method. The phylogenetic tree depicts the separation of the Hes1, Hes5, and Hes7 clusters. The symbol beside each gene shows their expression dynamics in the PSM, which is unclear specifically for her6 as it is expressed only during the 3-5 somite stage during fish segmentation.

Acknowledgments

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