The transcriptome results could be validated with qPCR, but downregulated amh and gsdf were observed in cyp17a1−/− fish after estradiol treatment from 16 to 23 dpf. (A,B) Relative expression of amh and gsdf in cyp17a1+/+ and cyp17a1−/− fish of PGC-rich and PGC-less groups at 23 dpf with qPCR. Upregulated expression of amh and gsdf was observed in cyp17a1−/− fish of PGC-rich and PGC-less groups at 23 dpf. (C,D) Relative expression of amh and gsdf in cyp17a1+/+ and cyp17a1−/− fish of PGC-rich and PGC-less groups treated with estradiol from 16 to 23 dpf. E2, 17 β-estradiol. Different letters in the bar charts represent significant differences.
Acknowledgments
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